bcl2  (Bioss)

Journal: bioRxiv

Article Title: Confirmatory evidence that miR-15a and miR-16 regulate BCL2 at the post-transcriptional level

doi: 10.64898/2026.03.02.708996

Figure Lengend Snippet: (A) Relative expression of miR-16-5p measured by RT-qPCR in MEG-01 wild-type (WT) cells transfected with miR-15a or miR-16-1 mimics, corresponding antisense oligonucleotides (AS), or their combinations. Data represent three independent experiments. **** p < 0.001. (B) Relative BCL2 mRNA expression in the same experimental conditions, determined by RT-qPCR using the 2^−ΔCt formula and actin as the reference gene. (C) Semi-quantitative RT-PCR analysis of BCL2 mRNA levels in MEG-01 cells under the indicated conditions, normalized to actin expression. (D) Western blot analysis of BCL2 protein expression in MEG-01 cells, with GAPDH used as a loading control.

Article Snippet: Membranes were blocked and incubated with a mouse monoclonal anti-BCL2 antibody (Santa Cruz Biotechnology) overnight at 4°C.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Western Blot, Control

Journal: Cancer Cell International

Article Title: Silencing Uracil-DNA glycosylase inhibits colorectal cancer progression

doi: 10.1186/s12935-025-04089-y

Figure Lengend Snippet: UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of caspase-9, and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars

Article Snippet: The membranes were incubated overnight at 4 °C with the following primary antibodies: UNG, P70 S6K, p-P70 S6K(S424) (1:1000, Bioworld), β-actin (1:1000, Abcam), cleaved caspase-3, AMPKα1/AMPKα2, p-AMPKα1(Thr183)/AMPKα2(Thr172) (1:1000, Beyotime), BAX, BCL2, AKT1/2/3, mTOR, and p-mTOR (Ser2448) (1:1000; BOSTER), cleaved caspase-9, p-AKT(Ser473), ERK1/2, and p-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) (1:1000; ZEN BIO), and GAPDH (1:1000; Proteintech).

Techniques: Protein-Protein interactions, Western Blot, Knockdown